Davids Biotechnologie GmbH
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Your custom antibody manufacturer since 1996
Davids Biotechnologie was founded 1996 in Regensburg, a world cultural heritage city in Germany. We offer all kind of custom antibody services. Polyclonal antibodies in rabbits, chicken, mice, rats or guinea pigs. Monoclonal Antibody development in mice or rats. Any kind of customer provided or Davids made antigens are possible. We developed animal and ecologically friendly protocols to produce the antibodies. In addition all antibodies are made directly from the manufacturer. This leads to efficient methods and low prices. Furthermore Davids developed methods to produce antibodies within 28 days (SuperFast) or with a very low amount of antigen (LowDose).
Our purification methods are very well established and we offer Affinity, ProteinA, Depletion, Size-Exclusion, Ion-Exchange, IgM or any other purification that fits to your antibody project.
We offer different kind of antigen production services. Peptide antibodies, protein expression, SDS-extractions and more to generate your antigens for the antibody production.
Additionally we modify your antibodies like fragmentation, enzyme or dye conjugations. In our Shop you can buy antibodies, proteins, kits or buffers.
Antibody Information - Issue III
SDS-Gel Antigen Purification
We are able to use your protein specific SDS-PAGE gel bands as antigen. The advantage is that you save time with complicated purification steps. In addition you are able to get rid of even small protein contaminations.
The gel extraction is simple. Prepare a solution with at least 0.5 mg protein for the immunization of one rabbit for example and additional 0.5 mg protein if you want to order an affinity purification. Just run multiple bands with your protein solution, dye the gel with coomassie and cut all bands that belongs to your protein from the SDS-PAGE gel. Send the gel pieces in a 2-ml or 15-ml tube at room temperature without the addition of any solvents to Davids Biotechnologie GmbH, Röntgenstrasse 3, 93055 Regensburg, Germany.
The final antibodies are usually very good for applications like western blot and all other fields where you need to detect the denaturated protein. Even though for some applications where you need to detect the protein in its native form the immunization of the native protein has advantages.
This time we focus peptide antibodies: We are able to produce antibodies against proteins you only know the sequence from. There is no need for protein production or purification. We help you to find peptides that represent your protein (see info box). These peptides have to be antigenic, soluble and must have good epitopes. For a higher chance to use the best peptide you can immunize one animal with two different peptides. This doubles your chance to use the best peptide for your applications.
The synthesized peptides are conjugated to a carrier molecule like KLH to improve the antigen presentation of the small peptides. The synthesis, conjugation and/or the immunizations can be performed by Davids.
We can produce peptide antibodies from rabbits, chicken, mice or other animals. Just send us your protein sequence (amino-acid one letter code prefered) and we analyze the sequence for free and handle your data confidentially. An order is only placed when you confirm one or more peptides. For further information go to our peptide section: Peptide Antibodies
Click here for the Peptide Antibody Scheme